The current study aims to search if the psychometric results proposed by Niñerola et al. (2006) for the Barriers for the Practice of Physical Activity Scale (BPPAS) are replicated in Mexican university students. A total of 1528 Mexican university students participated in this study (average age = 20.78 ± 2.45 years). The factor structure of the BPPAS was conducted by confirmatory factor analyses that showed a feasible and adequate fourfactor structure. The four factors (body image, fatigue, obligations, and environment), attending to statistical and susbstantive criteria, have shown fit indices of reliability and validity. Moreover, the factor structure, the standardized regression weights, and the intercepts were considered as invariants between athletic and non-athletic participants, obtaining differences in the factors punctuations. Future research should replicate these findings in wider samples.